| Catalog No. | HB011337 |
| Brand | abinScience |
| Host species | Mouse |
| Conjugation | APC |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. |
| Purity | >90% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM |
| Target | B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, CD22, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14 |
| References | 1. Kreitman RJ, et al. Complete regression of human B-cell lymphoma xenografts in mice treated with recombinant anti-CD22 immunotoxin RFB4(dsFv)-PE38 at doses tolerated by cynomolgus monkeys. Int J Cancer. 1999 Mar 31;81(1):148-55. [HB011337] 2. Mansfield E, et al. Recombinant RFB4 immunotoxins exhibit potent cytotoxic activity for CD22-bearing cells and tumors. Blood. 1997;90(5):2020-2026. [HB011337] |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P20273 |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Clone ID | RFB-4 |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |
Anti-Human Siglec-2/CD22 Recombinant Antibody (RFB-4), APC
Overview
Images
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD22 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD22 monoclonal antibody (Catalog: HB011337, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |