| Catalog No. | YP587013 | ||||||
| Brand | abinScience | ||||||
| Species reactivity | General | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Isotype | IgG | ||||||
| Applications | ELISA, IHC, WB | ||||||
| Target | Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A | ||||||
| References | 1. Shiba T,et al. Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. Biochim Biophys Acta. 1971 Sep 21;244(3):523-31. [YP587013] 2. ark MH, et al. Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. J Biol Chem. 2018 Nov 30;293(48):18710-18718. [YP587013] |
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| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | PubChem: 65396 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Clone ID | Hpu24 | ||||||
| Product Usage Information |
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| Note | For research use only. |
Anti-Hypusine Recombinant Antibody (Hpu24)
Overview
Images
 Bioactivity |
SEC-HPLC detection for Anti-Hypusine antibody (Hpu24). |
 SDS-PAGE |
SDS-PAGE for Anti-Hypusine antibody (Hpu24). |
 Western blot |
Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: NIH3T3 cell lysate Lane 5: Mouse pancears lysate Lane 7: Rat pancears lysate |
 Western blot |
Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: K562 cell lysate Lane 4: PC12 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: 293T cell lysate |