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Anti-Human Siglec-2/CD22 Reference Antibody (Epratuzumab, RUO)
Overview
Catalog No. HB011036
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-nd
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target Sialic acid-binding Ig-like lectin 2, Siglec-2, CD22, T-cell surface antigen Leu-14, BL-CAM, SIGLEC2, B-lymphocyte cell adhesion molecule, B-cell receptor CD22
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P20273
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name90Y-DOTA-hLL2, 90Y-epratuzumabtetraxetan, AMG-412, 90Y-hLL2, hLL2, 205923-57-5
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


SDS-PAGE		 SDS-PAGE for Research Grade Epratuzumab.

Flow cytometry		 Flow-cytometry using APC anti-human CD22 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD22 monoclonal antibody (Catalog HB011036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using PE anti-human CD22 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD22 monoclonal antibody (Catalog HB011036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using FITC anti-human CD22 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD22 monoclonal antibody (Catalog HB011036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.