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Anti-Human CD45 Reference Antibody (Apamistamab, RUO)
Overview
Catalog No. HY195016
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P08575
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
Images


Bioactivity
Detects Human CD45/PTPRC in indirect ELISAs.

Flow Cytometry
Flow-cytometry using anti-human CD45 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD45 monoclonal antibody (Catalog HY195016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Research Grade Apamistamab