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Anti-Human Integrin alpha V/CD51 Reference Antibody (Abituzumab, RUO)
Overview
Catalog No. HY065016
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG2-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target MSK8, VNRA, Vitronectin receptor, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51, ITGAV, VTNR
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P06756
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameDI-17E6, EMD525797, 1105038-73-0
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


Bioactivity		 SEC-HPLC detection for Research Grade Abituzumab.

Flow Cytometry		 Flow-cytometry using anti-human CD51 antibody. A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD51 monoclonal antibody (Catalog HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for Anti-Human Integrin alpha V/CD51 Reference Antibody (Abituzumab, RUO)

Flow CytoMetry		 Flow-cytometry using APC anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using PE anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.