Anti-Human Coagulation factor III/Tissue Factor Reference Antibody (Tisotumab, RUO)

Catalog No.	HB058016
Brand	abinScience
Host species	Human
Tested applications	WB, FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P13726
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	HuMax-TF-ADC, TF-011-MMAE, HuMax-TF, 1418628-81-5
Note	For research use only. Not suitable for clinical or therapeutic use.

SEC-HPLC	SEC-HPLC detection for Research Grade Tisotumab.
Bioactivity	Detects CD142/F3/TF in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Tisotumab
Flow CytoMetry	Flow-cytometry using anti-human CD142 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD142 monoclonal antibody (Catalog: HB058016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-CD142/F3/TF monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human CD142/F3/TF (Catalog No: HB058011), 50ng Lane 2: recombinant human CD142/F3/TF (Catalog No: HB058011), 30ng Lane 3: recombinant human CD142/F3/TF (Catalog No: HB058011), 20ng Lane 4: recombinant human CD142/F3/TF (Catalog No: HB058011), 10ng
Flow cytometry	Flow-cytometry using APC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using PE anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using FITC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.