| Catalog No. | HY328010 | ||||||
| Brand | abinScience | ||||||
| Tested applications | WB, FCM | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Isotype | IgG1, kappa | ||||||
| Applications | ELISA, FCM, WB, Neutralization | ||||||
| Target | B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6 | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | P05231 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||||
| Clone ID | Iv0022 | ||||||
| Product Usage Information |
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| Note | For research use only. Not suitable for clinical or therapeutic use. | ||||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
Overview
Images
 Bioactivity |
SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244). |
 Flow Cytometry |
Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022). |
 Western blot |
Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng |