Recombinant Human IL6 Protein, C-His (HY328021) expressed in Mammalian Cells, spanning Val30-Met212. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P05231
蛋白长度
Val30-Met212
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
经测试应用
WB
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
24.67 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-6 (IL6) is a ~23 kDa protein. Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is the therapeutic target of tocilizumab (Actemra).
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human IL6 Protein, C-His
Bioactivity
Detects Ziltivekimab in indirect ELISAs.
Bioactivity
Detects Sirukumab in indirect ELISAs.
Bioactivity
Detects Olokizumab in indirect ELISAs.
Western blot
Detected by Anti-IL6 monoclonal antibody (Catalog No: HY328056)
Western blot
Detected by Anti-IL6 monoclonal antibody (Catalog No: HY309013)
Western blot
Detected by Anti-IL6 monoclonal antibody (Catalog No: HY328010)
Western blot
Detected by Anti-IL6 monoclonal antibody (Catalog No: HS856016)
Bioactivity
Detected by Vamikibart (Catalog No: HY328176) in indirect ELISA.
Bioactivity
Detected by Pacibekitug (Catalog No: HY328186) in indirect ELISA.
Bioactivity
Immobilized HY328021 Human IL6 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY583011 Human CD126/IL6R/IL-6RA Recombinant Protein (C-His), the EC 50 is 815 ng/mL.
Bioactivity
Immobilized HY328021 Human IL6 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc), the EC 50 is 162 ng/mL.
Bioactivity
Immobilized HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY328021 Human IL6 Recombinant Protein (C-His), the EC 50 is 115 ng/mL.
Bioactivity
Immobilized CY583021 Canine CD126/IL6R/IL-6RA Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY328021 Human IL6 Recombinant Protein (C-His), the EC 50 is 42.6 ng/mL.
