| Catalog No. | HV974063 | ||||||
| Brand | abinScience | ||||||
| Host species | Human | ||||||
| Tested applications | WB, FCM | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | VHH-hFc | ||||||
| Applications | ELISA, WB, FCM | ||||||
| Target | B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1 | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | < 10 EU/mg as determined by LAL method. | ||||||
| Accession | Q9NZQ7 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| Clone ID | SAA2018 | ||||||
| Product Usage Information |
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| Note | For research use only. | ||||||
| RRID | Anti-Human CD274/PD-L1/B7-H1 Antibody (SAA2018) (abinScience Cat# HV974063, RRID:AB_3727930) |
Anti-Human PD-L1 Recombinant Antibody (SAA2018)
Overview
Images
 Bioactivity |
Detects Human CD274/PD-L1/B7-H1 in indirect ELISAs. |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: HV974063) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-CD274/PD-L1/B7-H1 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 50ng Lane 2: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 30ng Lane 3: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 20ng Lane 4: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 10ng |
 SDS-PAGE |
SDS-PAGE for Anti-Human CD274/PD-L1/B7-H1 Antibody (SAA2018) |