Mouse PD-L1 Recombinant Protein (C-His) (MV974011)
概述
图片
参考文献
产品说明书
概述
货号
MV974011
品牌
abinScience
描述
Recombinant Mouse CD274/PD-L1/B7-H1 Protein, C-His (MV974011) expressed in Mammalian Cells, spanning Met1-His239. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q9EP73
蛋白长度
Met1-His239
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
经测试应用
WB
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~32 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi).
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1. Tamura, H. et al. (2001) Blood 97, 1809-16. PMID: 11238124
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2. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443
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3. Wang, S. et al. (2003) The Journal of experimental medicine 197, 1083-91. PMID: 12719480
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Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Mouse CD274/PD-L1/B7-H1 Protein, C-His
Bioactivity
Detects Mouse CD274/PD-L1/B7-H1 in indirect ELISAs.
Western blot
Detected by Anti-CD274/PD-L1/B7-H1 monoclonal antibody (Catalog No: HB769023)
Bioactivity
Detected by anti-mouse PDL1 antibody (Catalog No: MV974040) in indirect ELISA
Bioactivity
Detected by Iv0226 (Catalog No: MB651030) in indirect ELISA.
Bioactivity
Detected by C3 (Catalog No: MV974033) in indirect ELISA.
