Human Tau Recombinant Protein (C-His)

Catalog No.	HY086012
Brand	abinScience
Tested applications	WB
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Purity	>90% as determined by SDS-PAGE.
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level	Please contact with the lab for this information.
Expression system	E. coli
Accession	P10636
Protein length	Met1-Leu758
Nature	Recombinant
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative name	MAPTL, Paired helical filament-tau, MTBT1, Microtubule-associated protein tau, TAU, Neurofibrillary tangle protein, MAPT, PHF-tau
Species	Homo sapiens (Human)
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Predicted molecular weight	80.23 kDa
Note	For research use only.

SDS-PAGE	SDS-PAGE for Recombinant Human MAPT/Tau/PHF-tau Protein, C-His
Bioactivity	Detects Gosuranemab in indirect ELISAs.
Bioactivity	Detects Zagotenemab in indirect ELISAs.
Bioactivity	Detects Tilavonemab in indirect ELISAs.
Western blot	Detected by Anti-MAPT/Tau/PHF-tau monoclonal antibody (Catalog No: HY086013)
Bioactivity	Detected by Research Grade Anti-Human MAPT/Tau/PHF-tau (DC8E8) (Catalog No: HY086013) in indirect ELISA
Bioactivity	Detected by 2C5 (Catalog No: HY086243) in indirect ELISA.
Bioactivity	Detected by SAA2138 (Catalog No: HY086253) in indirect ELISA.