A distinct effector B cell population drives autoantibody production in SARS-CoV-2 infection
Immunity
Autoantibodies (autoAbs) are linked to mortality and Long COVID, yet their cellular origins remain unclear. We analyzed the INCOV cohort and identified 12 age- and sex-matched participants with varying autoAb abundance and integrated single-cell RNA-seq and ATAC-seq data from B cells, plasma proteomics, proteome-wide autoAb profiling, clinical data, and in vitro assays. AutoAb abundance inversely correlated with neutralizing IgG and declined as infection resolved, paralleling the contraction of atypical memory B cells (AtMs). In vitro, AtMs preferentially differentiated into autoAb-producing antibody-secreting cells upon TLR7/8 stimulation. CD11c + AtMs (double-negative 2, DN2s) in autoAb-high individuals exhibited increased TLR7 signaling, oxidative stress, and isotype switching, regulated by transcription factors T-bet and XBP1. Integrated genetic and genomic analyses showed that DN2s had the strongest enrichment for autoimmune trait heritability and inferred regulatory effects of autoimmune risk variants among B cell subsets. These findings identify DN2s as key precursors of autoAb-producing cells during SARS-CoV-2 infection.
Related products
| Catalog No. |
Product Name |
Description |
Target |
| MB996019 |
InVivo Plus Anti-Mouse CD19 Antibody (1D3) |
InVivo Plus Anti-Mouse CD19 Antibody (1D3) (MB996019) is a mouse monoclonal antibody detecting CD19 in FCM, in vivo B cell depletion in vivo CD19. Suitable for Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
|
B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CD19, Differentiation antigen CD19, T-cell surface antigen Leu-12 |
