E. coli
Benzonase Nuclease is cloned from Serratia marcescens and expressed in E.coli.
Mammalian cells
Beta-Hexosaminidase B (HEXB) is expressed in Mammalian.
Beta-Hexosaminidase B can release all types of GlcNAc(β1-x) units, including the bisecting GlcNAc structure in N-linked glycans.
Homo sapiens (Human)
E. coli
T5 Exonuclease is cloned from Escherichia phage T5 and expressed in E.coli.
E. coli
Endo S2/Endo-beta-N-acetylglucosaminidase is cloned from Streptococcus pyogenes and expressed in E.coli.
Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.
E. coli
TEV Protease is cloned from Tobacco etch virus (TEV) and expressed in E.coli.
TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ↓(S/G/A/M/C/H).
E. coli
Expression Systems, Proteomics, Glycan Sequencing, Recombinant Glycoprotein Expression, Glycoprotein Analysis
E. coli
Remove or cleave SUMO tag from the SUMO fusion protein
E. coli
CAT / Catalase is cloned from human cell lines and expressed in E.coli.
Proteomics
E. coli
PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
Removal of high mannose N-glycans from glycoproteins
E. coli
M-MLV is cloned from MoMLV and expressed in E.coli.
cDNA Synthesis, Reverse Transcription (cDNA Synthesis), RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR