E. coli
Benzonase Nuclease is cloned from Serratia marcescens and expressed in E.coli.
Mammalian cells
Beta-Hexosaminidase B (HEXB) is expressed in Mammalian.
Beta-Hexosaminidase B can release all types of GlcNAc(β1-x) units, including the bisecting GlcNAc structure in N-linked glycans.
Homo sapiens (Human)
E. coli
TEV Protease is cloned from Tobacco etch virus (TEV) and expressed in E.coli.
TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ↓(S/G/A/M/C/H).
E. coli
T5 Exonuclease is cloned from Escherichia phage T5 and expressed in E.coli.
E. coli
Endo S2/Endo-beta-N-acetylglucosaminidase is cloned from Streptococcus pyogenes and expressed in E.coli.
Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.
E. coli
IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.
Proteomics
E. coli
Expression Systems, Proteomics, Glycan Sequencing, Recombinant Glycoprotein Expression, Glycoprotein Analysis
E. coli
Remove or cleave SUMO tag from the SUMO fusion protein
E. coli
CAT / Catalase is cloned from human cell lines and expressed in E.coli.
Proteomics
E. coli
PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
Removal of high mannose N-glycans from glycoproteins