Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014) is a rabbit polyclonal antibody detecting Caspase-3 in ELISA, IHC, WB. Suitable for Human, Rat, Pig, Dog, and Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human CASP3/Caspase-3 (Ser29-His277).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively).
1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680
2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 20566630
3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375
4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844
5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120
Western blot analysis was performed using anti-CASP3 polyclonal antibody at 1ug/mL on various samples. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate
