Anti-Human GAPDH Polyclonal Antibody (HY030014)

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概述
图片
参考文献
产品说明书

概述

货号

HY030014

品牌

abinScience

描述

Anti-Human GAPDH Polyclonal Antibody (HY030014) is a rabbit polyclonal antibody detecting GAPDH in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human GAPDH (Met1-Glu335).

靶标

GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P04406, P16858, P04797

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.

1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794

2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585

3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094

4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158

5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-GAPDH polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: K562 cell lysate
Lane 5: PC-3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate

参考文献

产品说明书

Anti-Human GAPDH Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GAPDH Polyclonal Antibody (HY030014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GAPDH Polyclonal Antibody (HY030014)

概述

图片

参考文献

产品说明书

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