E. coli
EndoS is cloned from Streptococcus pyogenes and expressed in E.coli.
Expression Systems, Proteomics, Glycan Sequencing, Recombinant Glycoprotein Expression, Glycoprotein Analysis
Streptococcus pyogenes
E. coli
Lambda PP is cloned from Escherichia phage lambda and expressed in E.coli.
Remove phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins.
Escherichia phage lambda (Bacteriophage lambda)
E. coli
TEV Protease is cloned from Tobacco etch virus (TEV) and expressed in E.coli.
TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ↓(S/G/A/M/C/H).
Tobacco etch virus (TEV)
E. coli
T5 Exonuclease is cloned from Escherichia phage T5 and expressed in E.coli.
Escherichia phage T5 (Enterobacteria phage T5)
E. coli
Neuraminidase (Sialidase) is cloned from Clostridium perfringens and expressed in E. coli.
Neuraminidase (Sialidase) has been used to desialylate transferrin in order to study its isoforms in human serum.
Clostridium perfringens
E. coli
3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
HRV-14
Terminal Transferase (TdT) is expressed in E. coli.
Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Bovine
E. coli
PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
Removal of high mannose N-glycans from glycoproteins
Elizabethkingia miricola
Yeast
Enterokinase is cloned from bovine and expressed in Yeast.
Fusion Protein Cleavage,Protein Purification,Protein Digestion
Bovine
E. coli
IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.
Proteomics
Streptococcus pyogenes