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HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line (VK805818)

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  • 1 ml
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概述
货号VK805818
品牌abinScience
描述
HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line (VK805818) is a stable cell line expressing SARS-CoV-2 Nucleoprotein (N). Engineered for consistent, high-level target expression in research applications.
Highlights
  • ●Stable Expression — Engineered for consistent, high-level target expression.
Accession号P0DTC9
应用FCM
种属Viral (SARS-CoV-2)
靶标SARS-CoV-2 Nucleoprotein (N)
检测方法Colorimetric
样本类型Plasma, Serum
实验类型Quantitative
检测范围1.56 - 100 ng/mL
灵敏度0.25 ng/mL
回收率80-120%
性能Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
运输2-8 ℃
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

Nucleoprotein (SARS-CoV-2 Nucleoprotein (N)) is a ~45 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dinesh, DC. et al. (2020) PLoS pathogens 16, e1009100. PMID: 33264373
2. Nabeel-Shah, S. et al. (2022) iScience 25, 103562. PMID: 34901782
3. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
NoteFor research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.
图片

  • Western blot

    Untransfected cells and Transfected cells were tested with western blot.

参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line

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