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HEK293T Human VIPR1/VPAC1 Stable Cell Line (HB111818)

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  • 1 ml
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概述
货号HB111818
品牌abinScience
描述
HEK293T Human VIPR1/VPAC1 Stable Cell Line (HB111818) is a stable cell line expressing VIPR1/VPAC1. Engineered for consistent, high-level target expression in research applications.
Highlights
  • ●Stable Expression — Engineered for consistent, high-level target expression.
Accession号P32241
应用FCM
种属Human
靶标VIPR1/VPAC1
检测方法Colorimetric
样本类型Plasma, Serum
实验类型Quantitative
检测范围1.56 - 100 ng/mL
灵敏度0.25 ng/mL
回收率80-120%
性能Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
运输2-8 ℃
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

Vasoactive intestinal polypeptide receptor 1 (VIPR1/VPAC1) is a ~51 kDa protein. G protein-coupled receptor activated by the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (ADCYAP1/PACAP). Binds VIP and both PACAP27 and PACAP38 bioactive peptides with the following order of ligand affinity VIP = PACAP27 > PACAP38. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway.

1. Xu, Y. et al. (2022) Nature communications 13, 2272. PMID: 35477937
2. Piper, SJ. et al. (2022) Nature communications 13, 7013. PMID: 36385145
3. Couvineau, A. et al. (1994) Biochemical and biophysical research communications 200, 769-76. PMID: 8179610
NoteFor research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.
图片

  • Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human VIPR1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
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浓度
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g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
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V₁ (起始体积)
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工作液
C₂ (终浓度)
×
V₂ (终体积)

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HEK293T Human VIPR1/VPAC1 Stable Cell Line

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