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Anti-Mouse Tau Recombinant Antibody (TAU-5) (MY086013)

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规格:
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  • 100ug
  • 1mg
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概述
货号MY086013
品牌abinScience
种属反应性Bovine, Human
应用IF, IHC-P, IP, WB
宿主Mouse
克隆类型Monoclonal
同种型IgG1, kappa
克隆号TAU-5
经测试应用FCM
靶标MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU
内毒素水平< 10 EU/mg
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P10637
RRIDAnti-Mouse MAPT/Tau/PHF-tau Antibody (TAU-5) (abinScience Cat# MY086013, RRID:AB_3726999)
状态Liquid
保存溶液 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Microtubule-associated protein tau (MAPT) is a ~76 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

NoteFor research use only.
Isotype ControlMouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Mouse MAPT/Tau/PHF-tau Antibody (TAU-5)

  • Flow cytometry

    Flow-cytometry using anti-human Tau antibody. 5637 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Tau monoclonal antibody (Catalog MY086013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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