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Anti-South American/Mojave rattlesnake crotoxin Recombinant Antibody (Al0G) (ZA049013)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
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  • 参考文献

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概述
货号ZA049013
品牌abinScience
种属反应性Crotalus durissus terrificus, Crotalus scutulatus scutulatus
应用ELISA, FCM, IF, SPR, WB
宿主Mouse
克隆类型Monoclonal
同种型IgG2a
克隆号Al0G
靶标Phospholipase A2 crotoxin basic subunit CBc, CB1, CTX subunit CBc, svPLA2, 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Basic phospholipase A2 Mtx-b, svPLA2, 3.1.1.4, Mojave toxin basic chain, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 CB1, Crotalus durissus terrificus (South American rattlesnake), Crotalus scutulatus scutulatus (Mojave rattlesnake)
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P62022, P62023
RRID

Anti-South American/Mojave rattlesnake crotoxin Antibody (Al0G) (abinScience Cat# ZA049013, RRID:AB_3729020)
_x005F_x000D_

状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Phospholipase A2 crotoxin basic subunit CBc is a ~15 kDa protein. Heterodimer CA-CB: Crotoxin is a potent presynaptic neurotoxin that possesses phospholipase A2 (PLA2) activity and exerts a lethal action by blocking neuromuscular transmission. It consists of a non-covalent association of a basic and weakly toxic PLA2 subunit (CBa2, CBb, CBc, or CBd), with a small acidic, non-enzymatic and non-toxic subunit (CA1, CA2, CA3 or CA4). The complex acts by binding to a specific 48-kDa protein (R48) receptor located on presynaptic membranes, forming a transient ternary complex CA-CB-R48, followed by dissociation of the CA-CB complex and release of the CA subunit. At equilibrium, only the CB subunits remain associated with the specific crotoxin receptor. In addition to neurotoxicity, crotoxin has been found to exert myotoxicity, nephrotoxicity, and cardiovascular toxicity.

1. Sampaio, SC. et al. (2010) Toxicon : official journal of the International Society on Toxinology 55, 1045-60. PMID: 20109480
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-South American/Mojave rattlesnake crotoxin Antibody (Al0G)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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