Anti-Human SETD2 IP Recombinant Antibody (SAA0221) (HP214013)
概述
图片
参考文献
产品说明书
概述
货号
HP214013
品牌
abinScience
种属反应性
Human
应用
ELISA, IF, IP, IP-MS
宿主
Mouse
克隆类型
Monoclonal
同种型
IgG
克隆号
SAA0221
靶标
Protein-lysine N-methyltransferase SETD2, HYPB, Histone-lysine N-methyltransferase SETD2, KMT3A, SET2, KIAA1732, Huntingtin yeast partner B, HIF-1, Huntingtin-interacting protein B, Huntingtin-interacting protein 1, HIP-1, Lysine N-methyltransferase 3A, SET domain-containing protein 2, p231HBP, SETD2, HIF1, hSET2
内毒素水平
Please contact with the lab for this information.
纯度
>95% as determined by SDS-PAGE.
纯化方式
Protein A/G purified from cell culture supernatant.
Accession号
Q9BYW2
RRID
Anti-Human SETD2 IP Antibody (SAA0221) (abinScience Cat# HP214013, RRID:AB_3729755)
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:2000-1:20000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景
Histone-lysine N-methyltransferase SETD2 is a ~287 kDa protein. Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction.
1. Sun, XJ. et al. (2005) The Journal of biological chemistry 280, 35261-71. PMID: 16118227
2. Yoh, SM. et al. (2008) Genes & development 22, 3422-34. PMID: 19141475
3. Hahn, MA. et al. (2011) PloS one 6, e18844. PMID: 21526191
4. de Almeida, SF. et al. (2011) Nature structural & molecular biology 18, 977-83. PMID: 21792193
5. Zheng, W. et al. (2012) Journal of the American Chemical Society 134, 18004-14. PMID: 23043551
6. Yang, S. et al. (2016) Genes & development 30, 1611-6. PMID: 27474439
7. Yuan, W. et al. (2009) The Journal of biological chemistry 284, 15701-7. PMID: 19332550
8. Carvalho, S. et al. (2013) Nucleic acids research 41, 2881-93. PMID: 23325844
9. Li, F. et al. (2013) Cell 153, 590-600. PMID: 23622243
10. Carvalho, S. et al. (2014) eLife 3, e02482. PMID: 24843002
