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Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237) (VK002063)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号VK002063
品牌abinScience
种属反应性Sindbis virus (SINV)
应用ELISA
宿主Mouse
克隆类型Monoclonal
同种型IgG, kappa
克隆号SAA2237
表达系统Mammalian cells
靶标glycoprotein E2
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P03316
RRIDAnti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237) (abinScience Cat# VK002063, RRID:AB_3729847)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Structural polyprotein (glycoprotein E2) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725
2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066
3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018
4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660
5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569
6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274
7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418
8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418
9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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