Anti-Human Nectin-2/CD112 Recombinant Antibody (SAA0405) (HV456107)

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100ug

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货号	HV456107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, WB
宿主	Mouse
克隆类型	Monoclonal
同种型	IgG2a, kappa
克隆号	SAA0405
经测试应用	WB, FCM
靶标	Herpesvirus entry mediator B, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, HVEB, PVRL2 receptor-related protein 2, CD112, PRR2, Nectin-2, HveB, NECTIN2
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q92692
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Nectin-2 (CD112/PVRL2) is a ~57 kDa protein. Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. 1. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705 2. Warner, MS. et al. (1998) Virology 246, 179-89. PMID: 9657005
Note	For research use only.

Bioactivity

Detects CD112/Nectin-2/PVRL2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human Nectin2 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog # HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human Nectin2 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog # HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD112 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD112 monoclonal antibody (Catalog: HV456107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD112/Nectin-2/PVRL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD112/Nectin-2/PVRL2 (Catalog No: HV456011)
Lane 2: negative control

产品说明书

Anti-Human Nectin-2/CD112 Recombinant Antibody (SAA0405).pdf

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Anti-Human Nectin-2/CD112 Recombinant Antibody (SAA0405) (HV456107)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human Nectin-2/CD112 Recombinant Antibody (SAA0405) (HV456107)

概述

图片

参考文献

产品说明书

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