Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Sorbitol dehydrogenase (SORD) is a ~38 kDa protein. Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility.
1. Maret, W. et al. (1988) Biochemistry 27, 1622-8. PMID: 3365415
2. Pauly, TA. et al. (2003) Structure (London, England : 1993) 11, 1071-85. PMID: 12962626
3. Safi, SZ. et al. (2014) BioMed research international 2014, 801269. PMID: 25105142
4. Dewanjee, S. et al. (2018) European journal of pharmacology 833, 472-523. PMID: 29966615
5. Frenette, G. et al. (2006) Journal of andrology 27, 233-9. PMID: 16278369
