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Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014)

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  • 100ug
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概述
货号HX129014
品牌abinScience
描述
Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014) is a rabbit polyclonal antibody detecting POU5F1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Macaca mulatta, and Pan troglodytes.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human POU5F1/OCT3/OCT4 (Ser136-Ser289).
靶标Octamer-binding protein 4, Oct-4, OCT3, OCT4, OTF3, OTF-3, Octamer-binding transcription factor 3, POU5F1, Oct-3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3
纯化方式Purified by antigen affinity column.
Accession号Q01860
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

POU domain, class 5, transcription factor 1 (POU5F1/OCT3) is a ~38 kDa protein. Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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