Anti-HDAC1 Polyclonal Antibody (HC626014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HC626014

品牌

abinScience

描述

Anti-HDAC1 Polyclonal Antibody (HC626014) is a rabbit polyclonal antibody detecting HDAC1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human HDAC1 (Val183-Ala482).

靶标

Histone deacetylase 1, HDAC1, RPD3L1, HD1

纯化方式

Purified by antigen affinity column.

Accession号

Q13547

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Histone deacetylase 1 (HDAC1) is a ~55 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription.

1. Qiu, Y. et al. (2006) Molecular cell 22, 669-79. PMID: 16762839

2. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056

3. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810

4. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

5. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

6. Jelinic, P. et al. (2011) Molecular and cellular biology 31, 54-62. PMID: 21041482

8. Ray, S. et al. (2005) Gastroenterology 129, 1616-32. PMID: 16285960

9. Zheng, G. et al. (2006) Biochemical and biophysical research communications 339, 1069-75. PMID: 16337145

10. Hung, JJ. et al. (2006) Molecular and cellular biology 26, 1770-85. PMID: 16478997

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-HDAC1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate

参考文献

产品说明书

Anti-HDAC1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HDAC1 Polyclonal Antibody (HC626014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HDAC1 Polyclonal Antibody (HC626014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价