Anti-PKP1 Polyclonal Antibody (HC672014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HC672014

品牌

abinScience

描述

Anti-PKP1 Polyclonal Antibody (HC672014) is a rabbit polyclonal antibody detecting PKP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human PKP1 (Asn500-Asn742).

靶标

B6P, Plakophilin-1, Band 6 protein, PKP1

纯化方式

Purified by antigen affinity column.

Accession号

Q13835

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Plakophilin-1 (PKP1) is a ~82 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Plays a role in desmosome protein expression regulation and localization to the desmosomal plaque, thereby maintaining cell sheet integrity and anchorage of desmosomes to intermediate filaments. Required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, YAP1, PKP1 and YWHAG. Positively regulates differentiation of keratinocytes, potentially via promoting localization of DSG1 at desmosome cell junctions. Required for calcium-independent development and maturation of desmosome plaques specifically at lateral cell-cell contacts in differentiating keratinocytes.

1. Wolf, A. et al. (2013) Journal of cell science 126, 1832-44. PMID: 23444369

2. Hofmann, I. et al. (2000) Journal of cell science 113 ( Pt 13), 2471-83. PMID: 10852826

3. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537

4. McGrath, JA. et al. (1997) Nature genetics 17, 240-4. PMID: 9326952

5. Sobolik-Delmaire, T. et al. (2010) The Journal of investigative dermatology 130, 2638-46. PMID: 20613778

6. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963

7. Fischer-Kešo, R. et al. (2014) Molecular and cellular biology 34, 4244-56. PMID: 25225333

Note

For research use only.

图片

参考文献

产品说明书

Anti-PKP1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PKP1 Polyclonal Antibody (HC672014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PKP1 Polyclonal Antibody (HC672014)

概述

图片

参考文献

产品说明书

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