Anti-SETDB1 Polyclonal Antibody (HC332014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HC332014

品牌

abinScience

描述

Anti-SETDB1 Polyclonal Antibody (HC332014) is a rabbit polyclonal antibody detecting SETDB1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human SETDB1 (Met1-Ser350).

靶标

H3-K9-HMTase 4, ESET, SETDB1, SET domain bifurcated 1, Histone H3-K9 methyltransferase 4, KMT1E, KIAA0067, Lysine N-methyltransferase 1E, ERG-associated protein with SET domain, Histone-lysine N-methyltransferase SETDB1

纯化方式

Purified by antigen affinity column.

Accession号

Q15047

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Histone-lysine N-methyltransferase SETDB1 is a ~143 kDa protein. Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.

1. Schultz, DC. et al. (2002) Genes & development 16, 919-32. PMID: 11959841

2. Ayyanathan, K. et al. (2003) Genes & development 17, 1855-69. PMID: 12869583

3. Wang, H. et al. (2003) Molecular cell 12, 475-87. PMID: 14536086

4. Sun, L. et al. (2016) Molecular cell 62, 958-966. PMID: 27237050

5. Chang, YH. et al. (2024) Cell reports 43, 114536. PMID: 39096901

6. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

7. Timms, RT. et al. (2016) Cell reports 17, 653-659. PMID: 27732843

9. Valle-García, D. et al. (2016) Epigenetics 11, 398-414. PMID: 27029610

Note

For research use only.

图片

参考文献

产品说明书

Anti-SETDB1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SETDB1 Polyclonal Antibody (HC332014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SETDB1 Polyclonal Antibody (HC332014)

概述

图片

参考文献

产品说明书

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