Anti-UBE2S Polyclonal Antibody (HS820014)

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概述
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参考文献
产品说明书

概述

货号

HS820014

品牌

abinScience

描述

Anti-UBE2S Polyclonal Antibody (HS820014) is a rabbit polyclonal antibody detecting UBE2S in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Rat, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human UBE2S (Met1-Ala209).

靶标

Ubiquitin-protein ligase S, E2EPF, Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-conjugating enzyme E2-24 kDa, E2 ubiquitin-conjugating enzyme S, Ubiquitin carrier protein S, UBE2S

纯化方式

Purified by antigen affinity column.

Accession号

Q16763

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Ubiquitin-conjugating enzyme E2 S (UBE2S) is a ~23 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo.

1. Garnett, MJ. et al. (2009) Nature cell biology 11, 1363-9. PMID: 19820702

2. Williamson, A. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 18213-8. PMID: 19822757

3. Sheng, Y. et al. (2012) Molecular & cellular proteomics : MCP 11, 329-41. PMID: 22496338

4. Brown, NG. et al. (2016) Cell 165, 1440-1453. PMID: 27259151

5. Kuang, P. et al. (2016) Scientific reports 6, 37441. PMID: 27910872

7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

8. Bremm, A. et al. (2010) Nature structural & molecular biology 17, 939-47. PMID: 20622874

Note

For research use only.

图片

参考文献

产品说明书

Anti-UBE2S Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-UBE2S Polyclonal Antibody (HS820014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-UBE2S Polyclonal Antibody (HS820014)

概述

图片

参考文献

产品说明书

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