Anti-CANT1 Polyclonal Antibody (HV161014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HV161014

品牌

abinScience

描述

Anti-CANT1 Polyclonal Antibody (HV161014) is a rabbit polyclonal antibody detecting CANT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human CANT1 (Ile107-Phe400).

靶标

CANT1, Putative NF-kappa-B-activating protein 107, Apyrase homolog, Soluble calcium-activated nucleotidase 1, SCAN-1, SHAPY, Putative MAPK-activating protein PM09

纯化方式

Purified by antigen affinity column.

Accession号

Q8WVQ1

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Soluble calcium-activated nucleotidase 1 (CANT1) is a ~44 kDa protein. Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis.

1. Smith, TM. et al. (2002) Archives of biochemistry and biophysics 406, 105-15. PMID: 12234496

2. Dai, J. et al. (2004) Cell 116, 649-59. PMID: 15006348

3. Yang, M. et al. (2004) Biochemistry 43, 9185-94. PMID: 15248776

4. Yang, M. et al. (2006) The Journal of biological chemistry 281, 28307-17. PMID: 16835225

5. Nizon, M. et al. (2012) Human mutation 33, 1261-6. PMID: 22539336

Note

For research use only.

图片

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CANT1 (HV161014) at a concentration of 26 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CANT1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

参考文献

产品说明书

Anti-CANT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CANT1 Polyclonal Antibody (HV161014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CANT1 Polyclonal Antibody (HV161014)

概述

图片

参考文献

产品说明书

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