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Anti-HDAC10 Polyclonal Antibody (HV042014)

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概述
货号HV042014
品牌abinScience
描述
Anti-HDAC10 Polyclonal Antibody (HV042014) is a rabbit polyclonal antibody detecting HDAC10 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human HDAC10 (Met1-Gln358).
靶标HDAC10, Polyamine deacetylase HDAC10, HD10, Histone deacetylase 10
纯化方式Purified by antigen affinity column.
Accession号Q969S8
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Polyamine deacetylase HDAC10 is a ~71 kDa protein. Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak.

1. Hai, Y. et al. (2017) Nature communications 8, 15368. PMID: 28516954
2. Kao, HY. et al. (2002) The Journal of biological chemistry 277, 187-93. PMID: 11677242
3. Guardiola, AR. et al. (2002) The Journal of biological chemistry 277, 3350-6. PMID: 11726666
4. Fischer, DD. et al. (2002) The Journal of biological chemistry 277, 6656-66. PMID: 11739383
5. Tong, JJ. et al. (2002) Nucleic acids research 30, 1114-23. PMID: 11861901
6. Radhakrishnan, R. et al. (2015) The Journal of biological chemistry 290, 22795-804. PMID: 26221039
7. Oehme, I. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E2592-601. PMID: 23801752
8. Ridinger, J. et al. (2018) Scientific reports 8, 10039. PMID: 29968769
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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