Anti-SMAD5 Polyclonal Antibody (HV175014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HV175014

品牌

abinScience

描述

Anti-SMAD5 Polyclonal Antibody (HV175014) is a rabbit polyclonal antibody detecting SMAD5 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Coturnix japonica.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human SMAD5 (Gly210-Val464).

靶标

SMAD 5, Mothers against decapentaplegic homolog 5, SMAD5, Mothers against DPP homolog 5, Smad5, SMAD family member 5, MAD homolog 5, JV5-1, MADH5, hSmad5

纯化方式

Purified by antigen affinity column.

Accession号

Q99717

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

SMAD family member 5 (SMAD5) is a ~52 kDa protein. Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis.

1. Fuchs, O. et al. (2002) Blood cells, molecules & diseases 28, 221-33. PMID: 12064918

2. Haag, J. et al. (2006) Experimental cell research 312, 1153-63. PMID: 16516194

3. Nishimura, R. et al. (1998) The Journal of biological chemistry 273, 1872-9. PMID: 9442019

4. Ruiz, L. et al. (2021) Computational and structural biotechnology journal 19, 632-646. PMID: 33510867

5. Fang, Y. et al. (2017) Cell research 27, 1083-1099. PMID: 28675158

Note

For research use only.

图片

参考文献

产品说明书

Anti-SMAD5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SMAD5 Polyclonal Antibody (HV175014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SMAD5 Polyclonal Antibody (HV175014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价