Anti-NUP85 Polyclonal Antibody (HV235014)

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概述
图片
参考文献
产品说明书

概述

货号

HV235014

品牌

abinScience

描述

Anti-NUP85 Polyclonal Antibody (HV235014) is a rabbit polyclonal antibody detecting NUP85 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human NUP85 (Arg130-Leu368).

靶标

Pericentrin-1, Nucleoporin Nup85, Nucleoporin Nup75, PCNT1, 85 kDa nucleoporin, NUP75, NUP85, Nuclear pore complex protein Nup85, FROUNT

纯化方式

Purified by antigen affinity column.

Accession号

Q9BW27

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Nuclear pore complex protein Nup85 is a ~75 kDa protein. Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade.

1. Harel, A. et al. (2003) Molecular cell 11, 853-64. PMID: 12718872

2. Orjalo, AV. et al. (2006) Molecular biology of the cell 17, 3806-18. PMID: 16807356

3. Terashima, Y. et al. (2005) Nature immunology 6, 827-35. PMID: 15995708

4. Braun, DA. et al. (2018) The Journal of clinical investigation 128, 4313-4328. PMID: 30179222

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-NUP85 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HT-29 cell lysate
Lane 3: LO2 cell lysate
Lane 4: 293T cell lysate

参考文献

产品说明书

Anti-NUP85 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NUP85 Polyclonal Antibody (HV235014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NUP85 Polyclonal Antibody (HV235014)

概述

图片

参考文献

产品说明书

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