| 货号 | HV095014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 品牌 | abinScience | ||||||||
| 种属反应性 | Human, Mouse, Rat, Bos taurus, Macaca fascicularis, Pongo abelii | ||||||||
| 应用 | ELISA, IHC, WB | ||||||||
| 宿主 | Rabbit | ||||||||
| 同种型 | IgG | ||||||||
| 克隆类型 | Polyclonal | ||||||||
| 免疫原 | E. coli - derived recombinant Human CACYBP (Ala2-Phe228). | ||||||||
| 靶标 | S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein | ||||||||
| 纯化方式 | Purified by antigen affinity column. | ||||||||
| Accession号 | Q9HB71 | ||||||||
| 状态 | Liquid | ||||||||
| 保存溶液 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Note | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HCT116 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: 293T cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HL60 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Rat brain lysate
Lane 9: Rat liver lysate
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