Anti-ABCB6 Polyclonal Antibody (HV027014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HV027014

品牌

abinScience

描述

Anti-ABCB6 Polyclonal Antibody (HV027014) is a rabbit polyclonal antibody detecting ABCB6 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ABCB6 (Ile590-Arg842).

靶标

UMAT, ATP-binding cassette sub-family B member 6, mitochondrial, MTABC3, Mitochondrial ABC transporter 3, ABCB6, P-glycoprotein-related protein, Mt-ABC transporter 3, PRP, Ubiquitously-expressed mammalian ABC half transporter

纯化方式

Purified by antigen affinity column.

Accession号

Q9NP58

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ATP-binding cassette sub-family B member 6 (ABCB6) is a ~93 kDa protein. ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen. May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress. May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils. In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin. In addition may play a role in the transition metal homeostasis.

1. Paterson, JK. et al. (2007) Biochemistry 46, 9443-52. PMID: 17661442

2. Chavan, H. et al. (2013) The Journal of biological chemistry 288, 22658-69. PMID: 23792964

3. Fukuda, Y. et al. (2016) Nature communications 7, 12353. PMID: 27507172

4. Wang, C. et al. (2020) Protein science : a publication of the Protein Society 29, 2363-2374. PMID: 33007128

5. Mitsuhashi, N. et al. (2000) The Journal of biological chemistry 275, 17536-40. PMID: 10837493

6. Krishnamurthy, PC. et al. (2006) Nature 443, 586-9. PMID: 17006453

7. Bergam, P. et al. (2018) Journal of molecular biology 430, 3802-3818. PMID: 29940187

8. Chavan, H. et al. (2011) Toxicological sciences : an official journal of the Society of Toxicology 120, 519-28. PMID: 21266531

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ABCB6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HL60 cell lysate

参考文献

产品说明书

Anti-ABCB6 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ABCB6 Polyclonal Antibody (HV027014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ABCB6 Polyclonal Antibody (HV027014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价