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Anti-UPB1 Polyclonal Antibody (HV482014)

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  • 参考文献

  • 产品说明书

概述
货号HV482014
品牌abinScience
描述
Anti-UPB1 Polyclonal Antibody (HV482014) is a rabbit polyclonal antibody detecting UPB1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human UPB1 (Trp121-Glu384).
靶标UPB1, N-carbamoyl-beta-alanine amidohydrolase, Beta-ureidopropionase, Beta-alanine synthase, BUP1, BUP-1
纯化方式Purified by antigen affinity column.
Accession号Q9UBR1
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Beta-ureidopropionase (UPB1) is a ~43 kDa protein. Catalyzes a late step in pyrimidine degradation. Converts N-carbamoyl-beta-alanine (3-ureidopropanoate) into beta-alanine, ammonia and carbon dioxide. Likewise, converts N-carbamoyl-beta-aminoisobutyrate (3-ureidoisobutyrate) into beta-aminoisobutyrate, ammonia and carbon dioxide (Probable).

1. van Kuilenburg, AB. et al. (2012) Biochimica et biophysica acta 1822, 1096-108. PMID: 22525402
2. Nakajima, Y. et al. (2014) Journal of inherited metabolic disease 37, 801-12. PMID: 24526388
3. Van Kuilenburg, AB. et al. (1999) Analytical biochemistry 272, 250-3. PMID: 10415095
4. Vreken, P. et al. (1999) Biochimica et biophysica acta 1447, 251-7. PMID: 10542323
5. Sakamoto, T. et al. (2001) Journal of nutritional science and vitaminology 47, 132-8. PMID: 11508704
6. Maurer, D. et al. (2018) The Biochemical journal 475, 2395-2416. PMID: 29976570
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-UPB1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Rat kidney lysate
    Lane 3: Rat liver lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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