Anti-AK3 Polyclonal Antibody (HV747014)

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概述
图片
参考文献
产品说明书

概述

货号

HV747014

品牌

abinScience

描述

Anti-AK3 Polyclonal Antibody (HV747014) is a rabbit polyclonal antibody detecting AK3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human AK3 (Leu8-Pro227).

靶标

GTP:AMP phosphotransferase AK3, mitochondrial, AK 3, AK6, Adenylate kinase 3 alpha-like 1, AK3, AKL3L, AK3L1, Adenylate kinase 3

纯化方式

Purified by antigen affinity column.

Accession号

Q9UIJ7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

GTP:AMP phosphotransferase AK3, mitochondrial is a ~25 kDa protein. Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Could also use ITP as phosphate donor. Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).

1. Noma, T. et al. (2001) The Biochemical journal 358, 225-32. PMID: 11485571

2. Rogne, P. et al. (2020) Biochemistry 59, 3570-3581. PMID: 32822537

Note

For research use only.

图片

Western blot

Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: Mouse heart lysate
Lane 5: Mouse liver lysate
Lane 6: Mouse kidney lysate
Lane 7: Rat heart lysate
Lane 8: Rat liver lysate

参考文献

产品说明书

Anti-AK3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-AK3 Polyclonal Antibody (HV747014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-AK3 Polyclonal Antibody (HV747014)

概述

图片

参考文献

产品说明书

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