Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法
稀释比例
ELISA
1:2000-1:20000
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Piezo-type mechanosensitive ion channel component 1 (PIEZO1) is a ~286 kDa protein. Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain. Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Conductance to monovalent alkali ions is highest for K(+), intermediate for Na(+) and lowest for Li(+). Divalent ions except for Mn(2+) permeate the channel but more slowly than the monovalent ions and they also reduce K(+) currents.
1. Andolfo, I. et al. (2013) Blood 121, 3925-35, S1-12. PMID: 23479567
2. Albuisson, J. et al. (2013) Nature communications 4, 1884. PMID: 23695678
3. Gnanasambandam, R. et al. (2015) PloS one 10, e0125503. PMID: 25955826
4. Zhou, Z. et al. (2023) Science (New York, N.Y.) 381, 799-804. PMID: 37590348
5. McHugh, BJ. et al. (2010) Journal of cell science 123, 51-61. PMID: 20016066
6. Li, J. et al. (2014) Nature 515, 279-282. PMID: 25119035
7. Tsuchiya, M. et al. (2018) Nature communications 9, 2049. PMID: 29799007
