Anti-Human ATG4A Polyclonal Antibody (HP478014)

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概述
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产品说明书

概述

货号

HP478014

品牌

abinScience

描述

Anti-Human ATG4A Polyclonal Antibody (HP478014) is a rabbit polyclonal antibody detecting ATG4A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ATG4A (Met1-Val398).

靶标

hAPG4A, Cysteine protease ATG4A, ATG4A, AUTL2, Autophagin-2, APG4A, AUT-like 2 cysteine endopeptidase, Autophagy-related protein 4 homolog A, Autophagy-related cysteine endopeptidase 2

纯化方式

Purified by antigen affinity column.

Accession号

Q8WYN0

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Cysteine protease ATG4A is a ~45 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3.

1. Scherz-Shouval, R. et al. (2003) The Journal of biological chemistry 278, 14053-8. PMID: 12473658

2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651

4. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

5. Shu, CW. et al. (2011) Journal of biomolecular screening 16, 174-82. PMID: 21245471

6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004

7. Nguyen, N. et al. (2020) The Journal of biological chemistry 295, 13584-13600. PMID: 32732290

9. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106

10. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human ATG4A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATG4A Polyclonal Antibody (HP478014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATG4A Polyclonal Antibody (HP478014)

概述

图片

参考文献

产品说明书

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