Anti-Human TIA1 Polyclonal Antibody (HB883014)

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概述
图片
参考文献
产品说明书

概述

货号

HB883014

品牌

abinScience

描述

Anti-Human TIA1 Polyclonal Antibody (HB883014) is a rabbit polyclonal antibody detecting TIA1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TIA1 (Asp104-Trp181).

靶标

TIA1, T-cell-restricted intracellular antigen-1, RNA-binding protein TIA-1, TIA-1, Nucleolysin TIA-1 isoform p40, p40-TIA-1

纯化方式

Purified by antigen affinity column.

Accession号

P31483

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Cytotoxic granule associated RNA binding protein TIA1 is a ~42 kDa protein. RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences. Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing. Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1. Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence. By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP.

1. Förch, P. et al. (2000) Molecular cell 6, 1089-98. PMID: 11106748

2. Förch, P. et al. (2002) The EMBO journal 21, 6882-92. PMID: 12486009

3. Izquierdo, JM. et al. (2007) The Journal of biological chemistry 282, 19410-7. PMID: 17488725

4. Dember, LM. et al. (1996) The Journal of biological chemistry 271, 2783-8. PMID: 8576255

7. Zuccato, E. et al. (2004) The Journal of biological chemistry 279, 16980-8. PMID: 14966131

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-TIA1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: Mouse brain lysate
Lane 5: Rat brain lysate

参考文献

产品说明书

Anti-Human TIA1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TIA1 Polyclonal Antibody (HB883014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TIA1 Polyclonal Antibody (HB883014)

概述

图片

参考文献

产品说明书

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