Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

HW292014

品牌

abinScience

描述

Anti-Human ATP6V1A Polyclonal Antibody (HW292014) is a rabbit polyclonal antibody detecting ATP6V1A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ATP6V1A (Lys456-Asp617).

靶标

ATP6V1A1, ATP6A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69 kDa subunit, V-ATPase subunit A, ATP6V1A, Vacuolar proton pump subunit alpha, Vacuolar ATPase isoform VA68

纯化方式

Purified by antigen affinity column.

Accession号

P38606

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

V-type proton ATPase catalytic subunit A (ATP6V1A) is a ~68 kDa protein. Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.

1. van Hille, B. et al. (1993) The Journal of biological chemistry 268, 7075-80. PMID: 8463241

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

3. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

4. Fassio, A. et al. (2018) Brain : a journal of neurology 141, 1703-1718. PMID: 29668857

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ATP6V1A polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: U87 cell lysate
Lane 4: Daudi cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse kidney lysate
Lane 8: Rat brain lysate

参考文献

产品说明书

Anti-Human ATP6V1A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

概述

图片

参考文献

产品说明书

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