Anti-Human MAD2L1 Polyclonal Antibody (HC694014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HC694014

品牌

abinScience

描述

Anti-Human MAD2L1 Polyclonal Antibody (HC694014) is a rabbit polyclonal antibody detecting MAD2L1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human MAD2L1 (Met1-Asp205).

靶标

MAD2L1, Mitotic arrest deficient 2-like protein 1, HsMAD2, MAD2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A, MAD2

纯化方式

Purified by antigen affinity column.

Accession号

Q13257

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) is a ~23 kDa protein. Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

1. Luo, X. et al. (2004) Nature structural & molecular biology 11, 338-45. PMID: 15024386

2. Ji, W. et al. (2018) The Journal of biological chemistry 293, 484-496. PMID: 29162720

3. Luo, X. et al. (2000) Nature structural biology 7, 224-9. PMID: 10700282

4. Luo, X. et al. (2002) Molecular cell 9, 59-71. PMID: 11804586

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human MAD2L1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAD2L1 Polyclonal Antibody (HC694014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAD2L1 Polyclonal Antibody (HC694014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价