Anti-Human SDHB Polyclonal Antibody (HB260014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

HB260014

品牌

abinScience

描述

Anti-Human SDHB Polyclonal Antibody (HB260014) is a rabbit polyclonal antibody detecting SDHB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human SDHB (Arg38-Val280).

靶标

Ip, SDH1, SDHB, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDH

纯化方式

Purified by antigen affinity column.

Accession号

P21912

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) is a ~31 kDa protein. Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.

1. Ardissone, A. et al. (2015) Molecular genetics and metabolism reports 5, 51-54. PMID: 26925370

2. Grønborg, S. et al. (2017) JIMD reports 33, 69-77. PMID: 27604842

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-SDHB polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse heart lysate
Lane 4: Mouse liver lysate
Lane 5: Rat brain lysate
Lane 6: Rat heart lysate
Lane 7: Rat liver lysate

参考文献

产品说明书

Anti-Human SDHB Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SDHB Polyclonal Antibody (HB260014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SDHB Polyclonal Antibody (HB260014)

概述

图片

参考文献

产品说明书

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