Anti-Human SULT1E1 Polyclonal Antibody (HW729014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HW729014

品牌

abinScience

描述

Anti-Human SULT1E1 Polyclonal Antibody (HW729014) is a rabbit polyclonal antibody detecting SULT1E1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SULT1E1 (Met1-Ile294 ).

靶标

ST1E1, STE, EST-1, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring, Estrogen sulfotransferase

纯化方式

Purified by antigen affinity column.

Accession号

P49888

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Sulfotransferase 1E1 (SULT1E1) is a ~35 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine. May play a role in gut microbiota-host metabolic interaction.

1. Hempel, N. et al. (2000) Biochemical and biophysical research communications 276, 224-30. PMID: 11006110

2. Pedersen, LC. et al. (2002) The Journal of biological chemistry 277, 17928-32. PMID: 11884392

3. Falany, CN. et al. (1995) The Journal of steroid biochemistry and molecular biology 52, 529-39. PMID: 7779757

4. Cook, IT. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 2069-78. PMID: 19589875

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-SULT1E1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse small intestine lysate

参考文献

产品说明书

Anti-Human SULT1E1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SULT1E1 Polyclonal Antibody (HW729014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SULT1E1 Polyclonal Antibody (HW729014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价