Anti-Human SSBP1 Polyclonal Antibody (HC422014)

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概述
图片
参考文献
产品说明书

概述

货号

HC422014

品牌

abinScience

描述

Anti-Human SSBP1 Polyclonal Antibody (HC422014) is a rabbit polyclonal antibody detecting SSBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SSBP1 (His16-Glu148).

靶标

Single-stranded DNA-binding protein, mitochondrial, Mt-SSB, MtSSB, PWP1-interacting protein 17, SSBP1, SSBP

纯化方式

Purified by antigen affinity column.

Accession号

Q04837

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Single-stranded DNA-binding protein, mitochondrial (SSBP1) is a ~17 kDa protein. Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.

1. Oliveira, MT. et al. (2011) The Journal of biological chemistry 286, 40649-58. PMID: 21953457

2. van Loon, B. et al. (2013) DNA repair 12, 177-87. PMID: 23290262

3. Del Dotto, V. et al. (2020) The Journal of clinical investigation 130, 108-125. PMID: 31550240

4. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372

5. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

6. Ciesielski, GL. et al. (2015) The Journal of biological chemistry 290, 28697-707. PMID: 26446790

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-SSBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: SW620 cell lysate

参考文献

产品说明书

Anti-Human SSBP1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SSBP1 Polyclonal Antibody (HC422014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SSBP1 Polyclonal Antibody (HC422014)

概述

图片

参考文献

产品说明书

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