Anti-Human MTHFD2 Polyclonal Antibody (HB832014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

HB832014

品牌

abinScience

描述

Anti-Human MTHFD2 Polyclonal Antibody (HB832014) is a rabbit polyclonal antibody detecting MTHFD2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MTHFD2 (Glu36-Asn350).

靶标

NMDMC, MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

纯化方式

Purified by antigen affinity column.

Accession号

P13995

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial (MTHFD2) is a ~37 kDa protein. Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.

Note

For research use only.

图片

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MTHFD2 (HB832014) at a concentration of 10.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MTHFD2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

参考文献

产品说明书

Anti-Human MTHFD2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MTHFD2 Polyclonal Antibody (HB832014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MTHFD2 Polyclonal Antibody (HB832014)

概述

图片

参考文献

产品说明书

相关产品推荐

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