Anti-Human VAPB Polyclonal Antibody (HF748014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HF748014

品牌

abinScience

描述

Anti-Human VAPB Polyclonal Antibody (HF748014) is a rabbit polyclonal antibody detecting VAPB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human VAPB (Met1-Glu124).

靶标

VAMP-B/VAMP-C, VAPB, VAP-B/VAP-C, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C

纯化方式

Purified by antigen affinity column.

Accession号

O95292

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Vesicle-associated membrane protein-associated protein B/C (VAPB) is a ~27 kDa protein. Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.

1. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433

2. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732

3. Kanekura, K. et al. (2006) The Journal of biological chemistry 281, 30223-33. PMID: 16891305

4. Chen, HJ. et al. (2010) The Journal of biological chemistry 285, 40266-81. PMID: 20940299

5. De Vos, KJ. et al. (2012) Human molecular genetics 21, 1299-311. PMID: 22131369

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-VAPB polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse muscle lysate
Lane 4: Mouse brain lysate
Lane 5: Mouse liver lysate
Lane 6: Mouse lung lysate
Lane 7: Rat muscle lysate
Lane 8: Rat brain lysate
Lane 9: Rat liver lysate
Lane 10: Rat lung lysate

参考文献

产品说明书

Anti-Human VAPB Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human VAPB Polyclonal Antibody (HF748014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human VAPB Polyclonal Antibody (HF748014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价