Anti-Human DNAJB11 Polyclonal Antibody (HP182014) is a rabbit polyclonal antibody detecting DNAJB11 in ELISA, IHC, WB. Suitable for Bovine, Dog, Human, Mouse, and Pongo abelii.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human DNAJB11 (Gly23-Glu333).
靶标
APOBEC1-binding protein 2, DNAJB11, DnaJ homolog subfamily B member 11, ERj3p, PWP1-interacting protein 4, ER-resident protein ERdj3, ABBP-2, DnaJ protein homolog 9, EDJ, ERJ3, Human DnaJ protein 9, HDJ9, HEDJ, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, ERdj3, Endoplasmic reticulum DNA J domain-containing protein 3, hDj-9
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
DnaJ homolog subfamily B member 11 (DNAJB11) is a ~40 kDa protein. As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.
1. Yu, M. et al. (2000) The Journal of biological chemistry 275, 24984-92. PMID: 10827079
2. Shen, Y. et al. (2005) Molecular biology of the cell 16, 40-50. PMID: 15525676
3. Cornec-Le Gall, E. et al. (2018) American journal of human genetics 102, 832-844. PMID: 29706351
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-DNAJB11 polyclonal antibody at 1μg/ml on various samples. Lane 1: Raji cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: 293T cell lysate Lane 6: MCF7 cell lysate Lane 7: NIH3T3 cell lysate Lane 8: Mouse heart lysate Lane 9: Mouse liver lysate Lane 10: Mouse lung lysate Lane11: Rat heart lysate Lane12: Rat liver lysate Lane13: Rat lung lysate
