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Anti-Human LMNB2 Polyclonal Antibody (HX984014)

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  • 参考文献

  • 产品说明书

概述
货号HX984014
品牌abinScience
描述
Anti-Human LMNB2 Polyclonal Antibody (HX984014) is a rabbit polyclonal antibody detecting LMNB2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human LMNB2 (Gly461-Ser581).
靶标LMN2, LMNB2, Lamin-B2
纯化方式Purified by antigen affinity column.
Accession号Q03252
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Lamin-B2 (LMNB2) is a ~69 kDa protein. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.

1. Parry, DA. et al. (2021) Genetics in medicine : official journal of the American College of Medical Genetics 23, 408-414. PMID: 33033404
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-LMNB2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: Raji cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Hela cell lysate
    Lane 5: 293T cell lysate
    Lane 6: MCF-7 cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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