Anti-Human SUCLA2 Polyclonal Antibody (HP689014)

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50ug
100ug

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概述
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参考文献
产品说明书

概述

货号

HP689014

品牌

abinScience

描述

Anti-Human SUCLA2 Polyclonal Antibody (HP689014) is a rabbit polyclonal antibody detecting SUCLA2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Columba livia, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SUCLA2 (Ser54-Glu445).

靶标

ATP-specific succinyl-CoA synthetase subunit beta, SCS-betaA, Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, A-SCS, Succinyl-CoA synthetase beta-A chain, SUCLA2

纯化方式

Purified by antigen affinity column.

Accession号

Q9P2R7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2) is a ~50 kDa protein. ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. Also able to act as an ATP-specific itaconyl- and malyl-CoA synthetase.

1. Elpeleg, O. et al. (2005) American journal of human genetics 76, 1081-6. PMID: 15877282

2. Marcero, JR. et al. (2021) Blood advances 5, 4831-4841. PMID: 34492704

3. Griffith, CM. et al. (2025) Nature chemical biology 21, 906-915. PMID: 40108300

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-SUCLA2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC3 cell lysate
Lane 4: LO2 cell lysate
Lane 5: Hela cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Mouse heart lysate
Lane 10: Mouse spleen lysate
Lane11: Rat brain lysate
Lane12: Rat liver lysate
Lane13: Rat heart lysate
Lane14: Rat spleen lysate

参考文献

产品说明书

Anti-Human SUCLA2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SUCLA2 Polyclonal Antibody (HP689014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SUCLA2 Polyclonal Antibody (HP689014)

概述

图片

参考文献

产品说明书

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